Welcome to the Bioinformatics Toolkit

The Bioinformatics Toolkit is a platform that integrates a great variety of tools for protein sequence analysis. Many tools are developed in-house, and serveral public tools are offered with extended functionality.

Most frequently used tools

HHpred Sensitive protein homology detection and structure prediction by HMM-HMM-comparison. Starting from a query sequence, HHpred builds a multiple sequence alignment using HHblits and turns it into a profile HMM. This is then compared it with a database of HMMs representing proteins with known structure (e.g. PDB, SCOP) or annotated protein families (e.g. PFAM, SMART, CDD, COGs, KOGs). The output is a list of closest homologs with alignments. HHpred can also build 3d homology models using the identified templates in the PDB database. It can optimize template picking and query-template alignments for homology modeling.
The HHblits software is part of the open source package HHsuite.

HHblits Remote homology detection method based on iterative HMM-HMM comparison. HHblits can build high-quality MSAs starting from single sequences or from MSAs. It transforms these into a query HMM and iteratively searches through uniprot20 or nr20 databases by adding significantly similar sequences from the previous search to the updated query HMM for the next search iteration. Compared to PSI-BLAST, HHblits is faster, up to twice as sensitive and produces more accurate alignments.
The HHblits software is part of the open source package HHsuite.

Quick2d Quick2D gives you an overview of secondary structure features like alpha-helices, extended beta-sheets, coiled coils, transmembrane helices and disorder regions. Predictions by PSIPRED, JNET, Prof(Rost), Prof(Ouali), Coils, MEMSAT2, HMMTOP, DISOPRED2 and VSL2.

Modeller A Program for Comparative Protein Structure Modelling by Satisfaction of Spatial Restraints.

Coils/PCoils This server compares a single sequence (COILS) or a sequence alignment (PCOILS) to a database of known coiled-coils and derives a similarity score. The program then calculates the probability that the sequence will adopt a coiled-coil conformation.

PSI-Blast Search with an amino acid sequence against protein databases for locally similar sequences. Similar to ProteinBLAST but more sensitive. PSI-BLAST first performs a BLAST search and builds an alignment from the best local hits. This alignment is then used as a query for the next round of search. After each successive round the search alignment is updated.

To find a tool not listed here, choose the appropriate section tab above, or look for the tool in the Quickfinder on the top right corner.


Recent Updates

June, 2013

The signal peptide identification protocol Predisi has been added to Quick2D.

April, 2013

The sequence alignment tool MSAProbs has been added to our library of programs.

April, 2013

Clustal Omega has been made default for the clustal alignment protocol .


Alternative Toolkit Location

You also can use our partner Bioinformatics Toolkit at the Max-Planck Institue for Developmental Biology, Tuebingen

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Job submission

Each tool has a separate input page with a web form in which the user can input sequence data, upload sequence files, and specify options. All tools that take alignments as input accept the most widely used formats (FASTA, CLUSTAL, Stockholm and A3M). You may also choose your own job-names to organize your work. Snail symbols inside the submit buttons inform you about tools that typically run for more than 10 minutes.


Job management

Located on the left of the screen is a sidebar pane that holds a status and section-coded list of all recent jobs in the current session. You can click on previously submitted jobs to check their status and view their results.
In general a session will last until the web browser is closed. However, because of different session implementations by the various browser types, jobs may occasionally disappear from the joblist. Therefore, noting down the job ids is recommended. Anonymous job results are stored two weeks, whereas jobs of logged-in users are stored at least two months.



Most of the tools in the Toolkit are interconnected, allowing job results of one tool to be forwarded as input to others. For example, you could run PSI-BLAST, parse out a multiple alignment of selected hits and send the results to a cluster analysis tool.


User databases

You may upload customized databases which are then accessible throughout the whole toolkit (upload once, use many times).

Reference: Biegert A, Mayer C, Remmert M, Söding J and Lupas A. The MPI Toolkit for protein sequence analysis Nucleic Acids Res. 34, W335-339 (download pdf)